Tlc analysis of a drug component

Thin layer chromotography techniques was performed on a sheet of glass or aluminium foil which is coated with thin layer adsorbent material usually silica gel.

Tlc analysis of a drug component

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March 4, Subject: Thin-Layer Chromatographic Analysis of Drug Components Experiment 15 The purpose of this experiment is to determine the components of an unknown drug and identify it as one of six 6 commercial drugs by using thin-layer chromatography.

I added approximately a quarter of a tablet of acetaminophen, aspirin, caffeine, ibuprofen, salicylamide, and the unknown 19 to separate test tubes containing 2. I noticed that my unknown was a coated tablet.

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Tlc analysis of a drug component

Each solution was spotted along a labeled starting line on the silica gel TLC plate. The TLC plate was put into a developing chamber containing When the TLC plate finished developing, I observed the plate under UV light and noticed that the salicylamide was the only spot that fluoresced purple while all the other spots fluoresced blue and I also noticed that the unknown had 2 spots.

I traced around the spots for each solution on the TLC plate, the only spot that I did not see was the one for the ibuprofen. I then placed it into an iodine chamber and noticed that the acetaminophen turned a yellowish color. I measured that the solvent traveled 64mm up the TLC plate by using the starting line as a reference, and, by using that information, I was able to calculate the Rf value of each solution by using: Commercial DrugRf Value Acetaminophen0.

I can speculate that the other substance is the coating from the tablet of the unknown.If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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Thin-Layer Chromatographic Analysis of Drug Component TLC is used because it is “sensitive, fast, simple and inexpensive analytical technique”2 (Mohrig ). The unknown solid (unknown #3 in this case) used in the experiment was obtained and added to a 2.

5ml of Ethanol/dichloromethane in a test tube. Abstract.


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Drug Analysis Using Thin-Layer Chromatography Annina Carter, Adirondack Community College, Queensbury, NY Description The majority of evidence submitted to crime labs comes from drug-related crimes. Often, this evidence includes unidentified powders that may be illegal drugs. In order to prosecute individuals.

The “compound” which is set to become the drug molecule undergoes safety tests and a series of experiments to prove that it is absorbed in the blood stream, distributed to proper site of action in the body, metabolized sufficiently and demonstrates its non-toxicity thus, can .

Tlc analysis of a drug component
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